Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor

This paper aims to reduce the gap between intensified innovative process designs and application of enzyme technologies at an industrial scale that often take full advantage microreactor systems. Soybean potato processing waste was used as source peroxidase in both free immobilized form for biodegradation Acid Violet 109 anthraquinone dye (AV109) a PTFE (polytetrafluoroethylene) which is 0.5 mm diameter 6 m length. High efficiencies 95 76% were achieved by soybean respectively after 3 min residence microreactor, with noticeable lack hydrogen peroxide inhibition. The reactor’s design, regard its length, significantly affected efficiency. polymer membrane formation cross-linking approach oxidized pectin or peel on inner wall SEM confirmed. efficiency peroxidases inversely correlated activity, hence highest recorded activity 11 19 ? mol/min peroxidase, respectively, initial concentration 0.05%. A promising reusability AV109 confirmed, since retained 65 35% tenth cycle, respectively. LC–MS GC–MS techniques confirmed AV dye, intermediates lower molecular weight separated identified. • peroxidase. Microreactor dye. Biodegradation 95% surface. enzymes cycle.